Leibniz Lecture by Prof. Joachim Spatz on 19 Nov 2018: Sequential Bottom-Up Assembly of Synthetic Cells

Monday, November 19, 2018 - 16:00
IIT Delhi, Seminar Hall

DFG India Office is pleased to announce that world-renowned scientist, Leibniz award holder and Max Planck Director Prof. Joachim Spatz (Max Planck Institute for Medical Research, Dept. of Cellular Biophysics, Heidelberg) will give a Leibniz Lecture on Mon, 19 November 2018 at 4 pm at the Seminar Hall of IIT Delhi entitled "Sequential Bottom-Up Assembly of Synthetic Cells".

Due to limited seating capacity, everyone interested in attending the lecture is politely requested to write to Charu.Relan@dfg.de or call Ms Relan under 011-4922-4901.

Abstract: The evolution of cellular compartments for spatially and temporally controlled assembly of biological processes became an essential step in developing life. Synthetic approaches towards cellular-like compartments are still lacking well-controlled functionalities as would be needed for more complex synthetic cells. In part, this is due to the mechanical and chemical instabilities of the lipid-based protocells and a lack of technical means for their well-controlled manipulation. We developed droplet supported lipid bilayer vesicles by microfluidics to generate mechanically and chemically stable and, therefore, manipulable cell-like compartments with a well-defined chemical and biophysical microenvironment. The enhanced stability enabled the sequential loading of such compartments with biomolecules by pico-injection microfluidics without compromising their functionality as synthetic cells. We demonstrate a successful sequential bottom-up assembly of a compartment with lipids, transmembrane proteins (integrin, FoF1-ATP synthase) and cytoskeleton proteins which would not assemble in a fully functional way by mixing and including them in one pot at once.